PCR Annealing Temperature Calculator
Biology
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This calculator determines the optimal annealing temperature for PCR based on primer sequences. The formula used is Tm = 2 * (A+T) + 4 * (G+C), where Tm is the melting temperature.
This calculator determines the optimal annealing temperature for PCR based on primer sequences. The formula used is Tm = 2 * (A+T) + 4 * (G+C), where Tm is the melting temperature.