Standard PCR
Concentration: 50 ng/µL; Volume: 1 µL; Molecular Weight of DNA/RNA: 650 daltons; Number of PCR Cycles: 30
Total Number of DNA/RNA Copies: 46,323,076,920,000 copies; Copies After PCR Cycles: 49,739,025,110,000,000,000,000 copies
DNA Copy Number Calculator
Biology0.0(0)
This calculator estimates the number of DNA or RNA copies in a solution or per PCR cycle using the concentration and volume of the sample.
More tools:Science & Lab Calculators
How To Use
How to use this calculator
Start with your values, or try a preset.
Step 1
Enter Concentration, Volume, Molecular Weight of DNA/RNA, and Number of PCR Cycles in the units shown.
Step 2
Read Total Number of DNA/RNA Copies and Copies After PCR Cycles in the results panel. Results update as you change the inputs.
Step 3
Choose a preset to load an example, then adjust the values for your situation.
Worked Examples
Example calculations
See the inputs and their calculated results.
High Concentration
Concentration: 200 ng/µL; Volume: 1 µL; Molecular Weight of DNA/RNA: 650 daltons; Number of PCR Cycles: 30
Total Number of DNA/RNA Copies: 185,292,307,700,000 copies; Copies After PCR Cycles: 198,956,100,400,000,000,000,000 copies
Low Volume
Concentration: 50 ng/µL; Volume: 0.5 µL; Molecular Weight of DNA/RNA: 650 daltons; Number of PCR Cycles: 30
Total Number of DNA/RNA Copies: 23,161,538,460,000 copies; Copies After PCR Cycles: 24,869,512,550,000,000,000,000 copies
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