Standard PCR
Concentration: 50 ng/µL; Volume: 1 µL; Molecular Weight of DNA/RNA: 650 daltons; Number of PCR Cycles: 30
Total Number of DNA/RNA Copies: 46,323,076,920,000 copies; Copies After PCR Cycles: 49,739,025,110,000,000,000,000 copies
DNA Copy Number Calculator
Biology0.0(0)
This calculator estimates the number of DNA or RNA copies in a solution or per PCR cycle using the concentration and volume of the sample.
More tools:Science & Lab Calculators
How To Use
How to use this calculator
Start with your values, or try a preset.
Step 1
Enter Concentration, Volume, Molecular Weight of DNA/RNA, and Number of PCR Cycles in the units shown.
Step 2
Read Total Number of DNA/RNA Copies and Copies After PCR Cycles in the results panel. Results update as you change the inputs.
Step 3
Choose a preset to load an example, then adjust the values for your situation.
Worked Examples
Example calculations
See the inputs and their calculated results.
High Concentration
Concentration: 200 ng/µL; Volume: 1 µL; Molecular Weight of DNA/RNA: 650 daltons; Number of PCR Cycles: 30
Total Number of DNA/RNA Copies: 185,292,307,700,000 copies; Copies After PCR Cycles: 198,956,100,400,000,000,000,000 copies
Low Volume
Concentration: 50 ng/µL; Volume: 0.5 µL; Molecular Weight of DNA/RNA: 650 daltons; Number of PCR Cycles: 30
Total Number of DNA/RNA Copies: 23,161,538,460,000 copies; Copies After PCR Cycles: 24,869,512,550,000,000,000,000 copies
Explore the topic
Explore More Science & Lab Calculators
Find calculators for chemistry, biology, lab dilution, concentration, osmolarity, genetics, physics, and experimental planning.
People Also Calculate
Related Calculators
Continue with another tool on a related topic.
Biology
Horse Weight Calculator
This calculator estimates a horse's weight based on its girth and body length measurements.
1,262 viewsOpen calculator
Biology
Trihybrid Cross Calculator - Punnett Square
Calculates the potential genotype combinations for three different traits using a Punnett square approach.
1,208 viewsOpen calculator
Biology
Log Reduction Calculator
Calculates the effectiveness of a treatment in reducing the number of microorganisms by measuring the log reduction value.
1,169 viewsOpen calculator
Biology
PCR Annealing Temperature Calculator
This calculator determines the optimal annealing temperature for PCR based on primer sequences. The formula used is Tm = 2 * (A+T) + 4 * (G+C), where Tm is the melting temperature.
1,168 viewsOpen calculator