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Serial Dilution Calculator

Biology
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Calculates the required volumes for performing serial dilutions to achieve a desired final concentration from a stock solution.

How To Use

How to use this calculator

Start with your values, or try a preset.

Step 1

Enter Stock Solution Concentration, Desired Final Concentration, Total Volume of Each Dilution, and Number of Serial Dilutions in the units shown.

Step 2

Read Dilution Factor, Individual Dilution Factor, Volume of Stock Solution Required, and Volume of Diluent Required in the results panel. Results update as you change the inputs.

Step 3

Choose a preset to load an example, then adjust the values for your situation.

Worked Examples

Example calculations

See the inputs and their calculated results.

100 to 1 Serial Dilution

Stock Solution Concentration: 100 mg/mL; Desired Final Concentration: 1 mg/mL; Total Volume of Each Dilution: 10 mL; Number of Serial Dilutions: 3

Dilution Factor: 100; Individual Dilution Factor: 4.64; Volume of Stock Solution Required: 2.16 mL; Volume of Diluent Required: 7.84 mL

1000 to 0.1 Serial Dilution

Stock Solution Concentration: 1,000 mg/mL; Desired Final Concentration: 0.1 mg/mL; Total Volume of Each Dilution: 10 mL; Number of Serial Dilutions: 4

Dilution Factor: 10,000; Individual Dilution Factor: 10; Volume of Stock Solution Required: 1 mL; Volume of Diluent Required: 9 mL

50 to 0.5 Serial Dilution

Stock Solution Concentration: 50 mg/mL; Desired Final Concentration: 0.5 mg/mL; Total Volume of Each Dilution: 10 mL; Number of Serial Dilutions: 2

Dilution Factor: 100; Individual Dilution Factor: 10; Volume of Stock Solution Required: 1 mL; Volume of Diluent Required: 9 mL

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