Standard DNA
Absorbance at 260nm: 1; Dilution Factor: 1; Extinction Coefficient (µg/mL): 50; Path Length (cm): 1
DNA Concentration: 50 µg/mL
DNA Concentration Calculator
Biology0.0(0)
This calculator determines the concentration of DNA, RNA, or custom oligonucleotides based on absorbance measurements at 260nm.
More tools:Science & Lab Calculators
How To Use
How to use this calculator
Start with your values, or try a preset.
Step 1
Enter Absorbance at 260nm, Dilution Factor, Extinction Coefficient (µg/mL), and Path Length (cm) in the units shown.
Step 2
Read DNA Concentration in the results panel. Results update as you change the inputs.
Step 3
Choose a preset to load an example, then adjust the values for your situation.
Worked Examples
Example calculations
See the inputs and their calculated results.
Standard RNA
Absorbance at 260nm: 1; Dilution Factor: 1; Extinction Coefficient (µg/mL): 40; Path Length (cm): 1
DNA Concentration: 40 µg/mL
High Concentration
Absorbance at 260nm: 2; Dilution Factor: 1; Extinction Coefficient (µg/mL): 50; Path Length (cm): 1
DNA Concentration: 100 µg/mL
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