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Ligation Calculator

Biology
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This calculator helps determine the ideal amounts of insert and vector DNA for ligation reactions based on their concentrations and desired molar ratios.

More tools:Science & Lab Calculators
How To Use

How to use this calculator

Start with your values, or try a preset.

Step 1

Enter Vector DNA Concentration, Insert DNA Concentration, Vector Size, Insert Size, and Molar Ratio (Insert:Vector) in the units shown.

Step 2

Read Vector Amount and Insert Amount in the results panel. Results update as you change the inputs.

Step 3

Choose a preset to load an example, then adjust the values for your situation.

Worked Examples

Example calculations

See the inputs and their calculated results.

Standard Ligation

Vector DNA Concentration: 50 ng/µL; Insert DNA Concentration: 50 ng/µL; Vector Size: 3,000 bp; Insert Size: 1,000 bp; Molar Ratio (Insert:Vector): 3

Vector Amount: 60 µL; Insert Amount: 60 µL

High Insert Ratio

Vector DNA Concentration: 100 ng/µL; Insert DNA Concentration: 100 ng/µL; Vector Size: 4,000 bp; Insert Size: 1,500 bp; Molar Ratio (Insert:Vector): 5

Vector Amount: 40 µL; Insert Amount: 75 µL

Low Vector Concentration

Vector DNA Concentration: 20 ng/µL; Insert DNA Concentration: 50 ng/µL; Vector Size: 2,500 bp; Insert Size: 800 bp; Molar Ratio (Insert:Vector): 2

Vector Amount: 125 µL; Insert Amount: 80 µL

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