Ideal Efficiency
Slope of the Standard Curve: -3.32
PCR Efficiency (%): 100.08
qPCR Efficiency Calculator
Biology0.0(0)
This calculator determines the PCR efficiency based on the slope of a standard curve obtained from quantitative PCR (qPCR) experiments.
More tools:Science & Lab Calculators
How To Use
How to use this calculator
Start with your values, or try a preset.
Step 1
Enter Slope of the Standard Curve in the units shown.
Step 2
Read PCR Efficiency (%) in the results panel. Results update as you change the inputs.
Step 3
Choose a preset to load an example, then adjust the values for your situation.
Worked Examples
Example calculations
See the inputs and their calculated results.
High Efficiency
Slope of the Standard Curve: -3
PCR Efficiency (%): 115.44
Low Efficiency
Slope of the Standard Curve: -3.6
PCR Efficiency (%): 89.57
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